Volume 2 Issue 1

Research Article: Effect of Dietary Acidolysis-Oxidized Konjac Glucomannan (A-OKGM) on Schizothorax Prenanti Immunity and Expression of Immune-Related Genes

Ming Rui Chen, Ying Long Wu*, Mei He, Zhen Zhen Lv, Jia Qi Zhao, Qiu Ping Yan and Li Mei Feng

The present study was conducted to investigate the effects of dietary Acidolysis-Oxidized Konjac Glucomannan (A-OKGM) (0, 0.4, 0.8 and 1.6 %) supplementation on the immunity and immune related genes expression of Schizothorax prenanti. The C - Reactive Protein (CRP) level was significantly higher than that in control group, regardless of the inclusion level. Similarly, the IgM level was significantly increased by all A-OKGM diets. The lysozyme activity in fish fed with 1.6% A-OKGM was markedly different from other groups. As for immune organ index, the 1.6% A-OKGM diet group significantly increased the spleen index. The liver index in fish fed with 0.8% A-OKGM diet was significantly higher than that of control group. The 0.4% and 1.6% A-OKGM diet groups significantly up-regulated TNFa gene expression in anterior gut. While the 0.8% and 1.6% A-OKGM diet groups also showed a significantly enhanced TNFa mRNA expression in mid and distal gut. The IL-1β gene expression in fish fed with 0.4% and 1.6% A-OKGM diet was significantly enhanced in anterior gut. The 0.8% and 1.6% A-OKGM diets significantly up-regualted the expression of IL-1β in distal gut. In a same pattern, the IL-6 mRNA level in 0.4% and 1.6% diet groups was significantly higher in anterior gut. The 0.8% and 1.6% A-OKGM diet groups significantly induced the IL-6 gene expression in distal gut. Present study suggested that A-OKGM can act as immunostimulant to improve the immune indexes and up-regulate the immune-related gene expressions.

Cite this Article: Chen MR, Wu YL, He M, Lv ZZ, Zhao JQ, et al. Effect of Dietary Acidolysis-Oxidized Konjac Glucomannan (A-OKGM) on Schizothorax Prenanti Immunity and Expression of Immune-Related Genes. Int J Vet Sci Technol. 2017;2(1): 040-047.

Published: 31 October 2017

Research Article: Detection of Pathogenic Leptospiraserovar in Malaysian Wild Rats by Real Time PCR based on LFB1 Gene

Latifah Ibrahim*, A Abdul Halim, H Roslina, M Y Aliza, H Asmah, Shafariatul Akmar, Rahmat MS and M Amal Nasir

Conventional laboratory diagnosis of Leptospirosis is laborious and time consuming. Real time PCR has been reported as cost effective for rapid diagnosis of leptospirosis. In this study, the Real Time PCR method based on LFB1 genes were used to detectpathogenic Leptospira spp in wild rats in selected location in Kuala Lumpur Malaysia (Chow Kit and Dato Keramat market). 140 rats were captured from that area, and samples from kidneys were collected for culture.All the isolates which were obtained were cultured in EMJH supplemented with 3 % rabbit serum. 4 reference genes which are Leptospiracanicola, Leptospirajavanica, Leptospirabataviae and Leptospirahardjo were used to monitor the detection of Leptospira spp. 25 samples were used including one negative sample to detect Leptospirasp. By using Agilent Brilliant III SYBR Master. Mix that consist a novel mutant Taq DNA polymerase which enhance higher nucleotide incorporation and a novel faster-activating hot start method which helps to minimize formation of primer-dimers and increase the specificity of target detection. The primers used were LFB1-F' and LFB1-R' for target detection. This gene has been reported to be responsible for the pathogenicity of Leptospira interrogans. All 4 reference gene were successfully amplified and all the 4 reference genes showed the same melting temperature which is 81°C. All amplified samples categorized into two genospecies as two distinct melting curve observed which was 81°C and 83.5°C respectively. In the real time PCR assay a positive reaction was detected by the accumulation of a fluorescent signal calculated in Ct (cycle threshold).All the control pathogenic leptospira produce Ct values ranging from 30.09 to 32.10. Twenty leptospira isolates from the wild rats gave Ct value ranging from 14.27 to 20.22. This study shows that Real Time PCR using the above primer set could be used to identify pathogenic leptospira from local wildrat's samples.

Cite this Article: Ibrahim L, Halim Menv AA, Ubil MIMLS ZE, Nadia MF, Asmah H, et al. Detection of Pathogenic Leptospiraserovar in Malaysian Wild Rats by Real Time PCR based on LFB1 Gene. Int J Vet Sci Technol. 2017;2(1): 035-038.

Published: 06 October 2017

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